Preparation and Purification of Epigallocatechin by High‐Speed Countercurrent Chromatography (HSCCC)

Abstract

Epigallocatechin (EGC) was prepared by the degallation of Epigallocatechin‐O‐3‐gallate (EGCG). EGCG was completely converted to EGC and gallic acid by adding 4 mg tannase/100 mg EGCG at the concentration of 2 mg/mL under pH 6.0, at 35°C for 30 min. EGC was then separated from the reaction mixture by HSCCC, using a two‐phase solvent system composed of hexane–ethyl acetate–water (1/9/10, v/v/v). Finally, 1.3 g of EGC at 97% purity was prepared from about 2.3 g of EGCG at 85% purity, indicating that EGC was almost completely recovered by HSCCC.

Keywords:

• Preparation
• Purification
• Epigallocatechin
• HSCCC