Two‐Dimensional Thin‐Layer Chromatography

Abstract

The two‐dimensional thin‐layer chromatography (2D TLC) technique is one of the more versatile methods of TLC development. The first application of the two‐dimensional chromatographic method to paper chromatography was reported in 1944 by Consden, Gordon, and Martin [Nyiredy, Sz.; Dallenbach‐Tölke, K.; Sticher, O. J. Planar Chromatogr. 1988, 1, 336]. Since that time, this method has been mostly used for the separation of a large number of compounds that cannot be separated in a single dimension TLC experiment. In 2D TLC, separation is on one surface spread along the entire area of the plate. The resolving power of the 2D TLC method has great application, especially in the areas of biochemistry, biology, natural products, pharmaceuticals, and environmental analysis.

^#Reprinted from the Encyclopedia of Chromatography (© 2003); Marcel Dekker, Inc., URL: http://www.dekker.com/servlet/product/productid/E‐ECHR.

Keywords:

• TLC
• 2D
• Prisma system
• UDM
• AMD
• Peptides

Notes

^#Reprinted from the Encyclopedia of Chromatography (© 2003); Marcel Dekker, Inc., URL: http://www.dekker.com/servlet/product/productid/E‐ECHR.