Abstract
High performance liquid chromatography (HPLC) is used extensively in the pharmaceutical industry due to the availability of fully automated systems, excellent quantitative precision, accuracy, broad linear dynamic range, and availability of a wide variety of column stationary phases. The technique is used in drug discovery, pre‐clinical, and clinical development and factory finished product analysis. HPLC has greatly developed through the years in terms of convenience, speed, increased selection of column stationary phases, high sensitivity, applicability to a broad variety of sample matrices, and ability to hyphenate the chromatographic method to spectroscopic detectors.
Acknowledgments
I would like to gratefully acknowledge Nelu Grinberg for his valuable technical discussions. I would also like to acknowledge and thank Vincent Antonucci, Xiaoyi Gong, Marie Achalabun, and Angela Pinto for their kind support and review of the document.