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Abstract
This review includes the latest references regarding modern and efficient thin layer chromatographic (TLC) methods like: gradient elution (GE) TLC, automated multiple development (AMD), and over‐pressured layer chromatography (OPLC). Detailed discussions are included about the mobile phase optimization by the “Prisma” method and other mathematical models. The analysis and quantification by photodensitometry of flavonoids, coumarins, saponins, alkaloids, and other classes of compounds include detailed experimental conditions, stationary and mobile phase, development methods, and quantitative determination by photodensitometry.