LC Determination of the Enantiomeric Purity of L‐Arginine Using a Teicoplanin Chiral Stationary Phase

Abstract

A direct enantioselective high‐performance liquid chromatography (HPLC) method was employed successfully for determination of the enantiomeric purity of L‐arginine. The elaborated method used teicoplanin macrocyclic antibiotic chiral stationary phase (CSP), known as Chirobiotic T, with a reversed‐phase mobile phase consisting of methanol:50 mM sodium dihydrogen phosphate buffer, pH 4.6 (2:8, v/v), at a flow rate of 1.0 mL/min and UV detection at 214 nm. Linearity, precision, accuracy, and the quantitation limit were determined. The method proved to be capable of determining 0.0025% (w/w) of D‐arginine (the enantiomeric impurity) contrary to the pharmacopoeial limit measurement, in which only amounts of D‐arginine higher than 1.5% (w/w) caused the measurement to fail.

Keywords:

• L‐Arginine
• Enantiomeric purity
• Chiral HPLC
• Chirobiotic T

Acknowledgment

The authors (H. Y. Aboul‐Enein and M. M. Hefnawy) would like to thank the King Faisal Specialist Hospital and Research Centre administration for their support for the Pharmaceutical Analysis Laboratory Research Program.