Determination of Phenylpropanoid Glycosides in Chinese Herbal Extracts from Pedicularis Species by HPLC

Abstract

A simple, rapid, and accurate high performance liquid chromatographic (HPLC) technique coupled with photodiode array (PDA) detection was developed for the simultaneous determination of three phenylpropanoid glycosides (PPGs), echinocoside (ECH), verbascoside (VER), pedicularioside A (PED‐A) in extracts of Pedicularis species. The optimized method was achieved for the separation and detection of selected constituents, using acetonitrile–water–acetic acid (20:80:1, v/v/v) as the mobile phase and 331 nm as the detection wavelength. The proposed method was employed to analyze the three different herbal medicines of Pedicularis species, named Pedicularis longiflora, P. longiflora var tubiformis, and P. kansuensis. The results demonstrate the differentiation of the contents of the three PPGs in the three herbal medicines.

Keywords:

• Pedicularis
• Phenylpropanoid glycosides
• Echinocoside
• Verbascoside
• Pedicularioside‐A
• High performance liquid chromatography

Acknowledgments

The work was supported by the “Bairen Jihua” Foundation of Chinese Academy of Sciences (CAS) in 2000 and National Natural Science Foundation of China (NSFC No. 20021001), as well as Natural Science Foundation of Gansu Province (No. ZS021‐A25‐011‐Z).