MOZPhCSE, a New Coumarin Based Fluorescent Derivatization Reagent

Abstract

The synthesis of a new fluorescent probe 2‐(3,7‐dioxo‐2‐phenyl‐3,7‐dihydrochromeno[7,6‐b][1,4]oxazin‐9‐yl)acetic acid succinimidyl ester was described. The reagent reacts easily with primary amines and forms highly fluorescent derivatives that can be separated and detected with an HPLC‐fluorometric detector. The new reagent was evaluated using pentylamine as a model compound. The separation was achieved on a C₁₈ column using a mixture of acetonitrile:ammonium acetate buffer as the mobile phase. Statistical evaluation of the methodology described reveals good linearity, precision and accuracy, and high sensitivity. Application of the methodology can be used for the separation of four aliphatic amines (pentylamine, hexylamine, octylamine, and decylamine).

Keywords:

• Coumarin
• Oxazin‐2‐one
• Derivatization
• Amine
• Fluorescent reagent
• MOZPhCSE