Qualitative and Quantitative Analysis by Hyphenated (HP)TLC‐FTIR Technique

Abstract

In recent years, much effort has been devoted to the coupling of thin layer chromatography (TLC) and high performance thin layer chromatography (HPTLC) with spectrometric methods because of the robustness and simplicity of use of (HP)TLC and the need for detection techniques that provide identification and determination of sample constituents. Infrared (IR) is one of the spectroscopic methods that have been coupled with (HP)TLC. IR spectroscopy has a high potential for the elucidation of molecular structures; the characteristic absorption bands can be used for compound specific detection. The (HP)TLC‐FTIR coupled method has been widely used in modern laboratories for qualitative and quantitative analysis. The potential and power of this method is demonstrated by its application in various fields of analysis, such as drug analysis, forensic analysis, food analysis, environmental analysis, biological analysis, etc. The hyphenated (HP)TLC‐FTIR technique will continue to be developed in the future with the aim of taking full advantage of this method's capabilities.

Keywords:

• Thin layer chromatography
• High performance thin layer chromato-graphy
• IR spectroscopy
• Hyphenated techniques
• Qualitative analysis
• Quantitative analysis