Amperometric Detection of Estradiol and its Metabolites in Menstrual Women and the Urine of Pregnant Rabbits

Abstract

A liquid chromatography method with electrochemical detection has been developed for the quantitative measurement for the five estrogen derivatives (estrone, estradiol, estriol, 2‐methoxyestrone, and ethinylestradiol) in human and rabbit urine. The detection cell consisting of a dual thin‐layer glassy carbon electrochemical signal was obtained with a supporting electrolyte containing 40% methanol–25% acetonitrile–35% phosphate buffer (pH 6.0) as the mobile phase. The method was applied for the determination of the four compounds in menstrual women and the urine of pregnant rabbits.

Keywords:

• Estradiol and its metabolites
• Biological urine
• Electrochemical detection

Acknowledgment

Financial support of this work (NSC 91‐2113‐M‐041‐004) by the National Science Council of the Republic of China is gratefully acknowledged.