Mixed‐Model of Affinity and Hydrophobic Interaction for Drug Retention in Cell Membrane Chromatography

Abstract

According to the stoichiometric displacement concept and some reasonable assumptions for the surface property and the interaction between the cell membrane stationary phase (CMSP) and drugs, a mixed model of affinity and hydrophobic interaction for drug retention on the CMSP is reported. An equation expressing the “mixed mechanism” was tested by five kinds of calcium antagonists and a rabbit myocardium CMSP with three kinds of different mobile phases. The experimental results basically coincide with that of the theoretical expectation.

Keywords:

• Cell membrane stationary phase
• Cell membrane chromatography
• Stoichiometric displacement model
• Retention mechanism
• Calcium antagonist

Acknowledgments

The authors thank professor Tonghui Zhou, member of Chinese Academy for useful advice in this study. Support of this work by the National Nature Science Foundation of China (No's. 39770856, 39880003, 20175016, and 30171080) is gratefully acknowledged.