Advanced search
Publication Cover
Journal of Liquid Chromatography & Related Technologies Volume 28, 2005 - Issue 12-13: Papers presented at CCC 2004-Tokyo August 28-31, 2004
Submit an article Journal homepage
106
Views
18
CrossRef citations to date
0
Altmetric
Original Articles

Analysis of Polyphenols from Hop Bract Region Using CCC

Masami Kurumatani Fundamental Research Laboratory, Asahi Breweries, Ltd., Moriya, Ibaraki, Japan
,
Rumi Fujita Fundamental Research Laboratory, Asahi Breweries, Ltd., Moriya, Ibaraki, Japan
,
Motoyuki Tagashira Fundamental Research Laboratory, Asahi Breweries, Ltd., Moriya, Ibaraki, JapanCorrespondence[email protected]
,
Toshihiko Shoji Fundamental Research Laboratory, Asahi Breweries, Ltd., Moriya, Ibaraki, Japan
,
Tomomasa Kanda Fundamental Research Laboratory, Asahi Breweries, Ltd., Moriya, Ibaraki, Japan
,
Mitsuo Ikeda Fundamental Research Laboratory, Asahi Breweries, Ltd., Moriya, Ibaraki, Japan
,
Atsushi Shoji Division of Structural Biology and Analytical Science, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan
,
Akio Yanagida Division of Structural Biology and Analytical Science, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan
,
Yoichi Shibusawa Division of Structural Biology and Analytical Science, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan
,
Heisaburo Shindo Division of Structural Biology and Analytical Science, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan
&
Yoichiro Ito Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute of Health, Bethesda, MD, USA
 show all
Pages 1971-1983 | Received 21 Sep 2004, Accepted 10 Nov 2004, Published online: 06 Feb 2007
 
Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days

Abstract

Polyphenols derived from hop (Humulus lupulus L.) bract region (HBP) can be used as food materials, thereby preventing dental caries. Chemical details of the active substances need to be elucidated. The polyphenols from hop bract (HBP) region were purified by countercurrent chromatography (CCC). The fractions were analyzed by high‐performance size‐exclusion chromatography (HPSEC) and reversed phase high‐performance liquid chromatography (RP‐HPLC). From HBP fractions by HPSEC, some low‐molecular‐ weight polyphenols (glycosides of flavonoids, catechins, and proanthocyanidins) were identified by RP‐HPLC. However, a very hydrophilic fraction was found to have the most potent cavity‐preventive activity, but it showed no peak in its RP‐HPLC chromatogram (absence of small polyphenols). HPSEC analysis showed that the major components of this fraction were high‐molecular weight substances, which were supposed to be proanthocyanidins, consisting of approximately 22 catechin units in its structure.

Log in via your institution

Access through your institution

Log in to Taylor & Francis Online

Restore content access

Restore content access for purchases made as guest
Purchase options * Save for later

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 583.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.