Preparative Separation of a Minor Active Chromone from Aloe vera Leaves by CCC

Abstract

HSCCC was applied successively in the preparative separation of a minor active chromone from an extract of Aloe vera leaves by combination with other traditional pretreatment, extraction, and partition methods. The chromone was finally identified as 8‐C‐β‐D‐(2′‐O‐(E)‐cinnamoyl) glycopyranosyl‐2‐(2‐hydroxy) propyl‐7‐methoxy‐5‐methyl‐chromone by FAB‐MS, ¹H NMR, and ¹³C NMR. Both of the solvent systems composed of the normal and reversed phase systems, such as CH₂Cl₂‐MeOH‐H₂O (5∶4∶2, v/v/v) and hexane‐ethyl acetate‐methanol‐water (1∶5∶1∶5, v/v/v/v), can be used for the preparative separation of the target chromone compound. The later solvent system was superior regarding sample loading capacity, environmental concerns (no chlorinated solvent), and peak resolution.

Keywords:

• Active chromone
• Aloe vera
• CCC
• Preparative separation

Acknowledgments

The authors thank National Natural Science Foundation of China and Beijing Municipal Education Committee for financial support. We also thank the National Center of Biomedical Analysis, Academy of Military Medical Sciences (Beijing) for their FAB‐MS and NMR analysis.