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Original Articles

Preparative Separation of a Minor Active Chromone from Aloe vera Leaves by CCC

, , , &
Pages 2005-2016 | Received 19 Sep 2004, Accepted 21 Dec 2004, Published online: 06 Feb 2007
 

Abstract

HSCCC was applied successively in the preparative separation of a minor active chromone from an extract of Aloe vera leaves by combination with other traditional pretreatment, extraction, and partition methods. The chromone was finally identified as 8‐C‐β‐D‐(2′‐O‐(E)‐cinnamoyl) glycopyranosyl‐2‐(2‐hydroxy) propyl‐7‐methoxy‐5‐methyl‐chromone by FAB‐MS, 1H NMR, and 13C NMR. Both of the solvent systems composed of the normal and reversed phase systems, such as CH2Cl2‐MeOH‐H2O (5∶4∶2, v/v/v) and hexane‐ethyl acetate‐methanol‐water (1∶5∶1∶5, v/v/v/v), can be used for the preparative separation of the target chromone compound. The later solvent system was superior regarding sample loading capacity, environmental concerns (no chlorinated solvent), and peak resolution.

Acknowledgments

The authors thank National Natural Science Foundation of China and Beijing Municipal Education Committee for financial support. We also thank the National Center of Biomedical Analysis, Academy of Military Medical Sciences (Beijing) for their FAB‐MS and NMR analysis.

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