UV‐Guided Isolation of Fungal Metabolites by HSCCC

Abstract

Analytical standardised reversed phase liquid chromatography (RPLC) data can be helpful in finding a suitable solvent combination for isolation of fungal metabolites by high‐speed counter current chromatography. Analysis of the distribution coefficient (K _D) of fungal metabolites in a series of biphasic systems consisting of n‐heptane–ethyl acetate (EtOAc)–methanol (MeOH)–water has been used to illustrate the relationship between the RPLC retention indexes and K _D's. The relationship can be used to select a suitable solvent combination for HSCCC, as demonstrated by the isolation of two new cyclic peptides from a crude fungal extract.

Keywords:

• CCC
• Filamentous fungi
• Psychrophilin
• LECA
• LC‐MS
• Dereplication
• Alkylphenones
• Margraff system
• Penicillium rivulum

Acknowledgments

This project was supported by the Danish Technical Research Council under the project “Functional biodiversity in Penicillium and Aspergillus” (grant no. 9901295). We are indebted to Jens C. Frisvad for identification of the fungal culture.