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Journal of Liposome Research Volume 11, 2001 - Issue 1
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Research Article

TRANSFECTION OF NORMAL HUMAN EPIDERMAL KERATINOCYTES WITH LIPID/DNA COMPLEXES IN VITRO

Sebastian Zellmer Institute of Biochemistry, Medical Faculty, University Leipzig, Leipzig, D-04103, Germany
,
Andrej Surovoy Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow, 117871, Russia
,
Frank Gaunitz Institute of Biochemistry, Medical Faculty, University Leipzig, Leipzig, D-04103, Germany
,
Jürgen Salvetter Institute of Anatomy, Medical Faculty, University Leipzig, Leipzig, D-04103, Germany
,
Rolf Gebhardt Institute of Biochemistry, Medical Faculty, University Leipzig, Leipzig, D-04103, Germany
&
Dieter Reissig Institute of Anatomy, Medical Faculty, University Leipzig, Leipzig, D-04103, Germany
Pages 43-54 | Published online: 30 Apr 2001
 
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Abstract

Highly proliferative normal human epidermal keratinocytes (NHK) were isolated from human foreskin biopsies, cultivated in serum-free medium and characterized by flow cytometry. The expression of cytokeratin 19, cytokeratin 14 and vimentin indicated that the suspension contained a high percentage of undifferentiated cells of the basal epidermal layer. The NHK were transfected in vitro with lipid/DNA complexes made of Effectene or Lipofectamine and different reporter genes. The transfection efficiency of Effectene/DNA complexes was 20fold higher compared to Lipofectamine. Transfected keratinocytes continued to grow and developed within 2 weeks a cellular multilayer (3-D culture). Areas of transfected cells were detected within this layer.

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