BINDING OF A PORPHYRIN CONJUGATE OF HOECHST 33258 TO DNA. II. NMR SPECTROSCOPIC STUDIES DETECT MULTIPLE BINDING MODES TO A 12-MER NONSELF-COMPLEMENTARY DUPLEX DNA

Abstract

We have probed by ¹H NMR spectroscopy the molecular basis of the interaction between Hoechst 33258 conjugated to a des-metalloporphyrin and a non self-complementary duplex DNA sequence, designed on the known chemical nuclease selectivity of this system. The imino NMR spectra are consistent with two distinct families of structure, that is, PORHOE binding either way along the duplex. 2D spectral, T ₂, and linewidth data suggest multiple species within the two conformational families.

Keywords:

• DNA-binding
• Minor groove
• Ligand
• NMR
• NOESY
• DNA
• duplex

ACKNOWLEDGMENTS

We are grateful to the EU for a Marie-Curie Postdoctoral Fellowship (SF), to the BBSRC for support (EVB), and to Dr. Martin Baron for 400 MHz NMR access.