Abstract
Fluorinated analogs of 2′- and 3′-deoxy-5′-methylthioadenosine 1–4 caused irreversible inactivation of AdoHcy hydrolase. Based on the ESI-Mass spectra analysis of the inactivated enzyme with the fluorinated analog 1 a mechanism of inactivation is proposed.
ACKNOWLEDGMENTS
The authors express their thanks to Pr. Michael S. Hershfield and Dr. Franscisco Arredondo (Duke University School of Medecine) for providing sample of E. Coli transformed with a plasmid encoding for human placental Ado Hcy hydrolase, Dr. Helene Rogniaux and Dr. Alain Van Dorsselaer (University Louis Pasteur, France) for carrying out the mass spectra analysis in this study.