NOVEL NUCLEOSIDE TRIPHOSPHATE ANALOGS FOR THE ENZYMATIC LABELING OF NUCLEIC ACIDS

Abstract

We have evaluated several novel nucleotide analogs suitable for enzymatic labeling of nucleic acid targets for a variety of array-based assays. Two new reagents in particular, a C4-labeled 1-(2′,3′-dideoxy-β-D-ribofuranosyl) imid- azole-4-carboxamide 5′-triphosphate 5 and an N1-labeled 5-(β-D- ribofuranosyl)-2,4(1H,3H)-pyrimidinedione 5′-triphosphate 3, were found to be excellent substrates for labeling with terminal deoxynucleotidyl transferase and T7 RNA polymerase, respectively.

ACKNOWLEDGMENTS

We would like to thank our Affymetrix colleagues Huu Tran, Hajime Matsuzaki, Huong Quang and Dr. Jian-bing Fan for assistance with GeneChip® data analysis. We also would like to acknowledge Morte Vaghefi and Kristin Anderson at TriLink Biotechnologies for contract synthesis support.