SYNTHESES OF DNA DUPLEXES CONTAINING A C‒C INTERSTRAND CROSS-LINK

Abstract

Short DNA duplexes that contain a N⁴C-ethyl-N⁴C interstrand cross-link were prepared on controlled pore glass supports using a DNA synthesizer. The C‒C cross-link was introduced via a convertible nucleoside on the support or by using a protected C‒C cross-link phosphoramidite. An orthogonal protection scheme allowed selective chain growth in either a 3′ → 5′ or 5′ → 3′ direction. The cross-linked duplexes were purified by HPLC and characterized by MALDI-TOF mass spectrometry and/or by enzymatic digestion.

ACKNOWLEDGEMENT

These studies were supported by a grant from the National Cancer Institute, CA082785.