DAUNOMYCIN BINDING TO DEOXYPOLYNUCLEOTIDES WITH ALTERNATING SEQUENCES: COMPLETE THERMODYNAMIC PROFILES OF HETEROGENEOUS BINDING SITES

ABSTRACT

Complete thermodynamic binding profiles for the interaction of the anticancer drug, daunomycin with natural DNA and synthetic deoxypolynucleotides were described. Fluorescence titration method was used to estimate the equilibrium binding constants. Binding isotherms were found to be surprisingly complex in some cases, presumably because there were heterogeneous sites even in simple deoxypolynucleotides of repeating sequence. Some polynucleotides consisting of alternating sequence contain at least two different binding sites for daunomycin. The binding affinity of the primary binding sites of alternating and non-alternating sequences was found to differ by two orders of magnitude. An isothermal microtitration calorimeter was used to directly measure the binding enthalpy at 25°C with a high sensitivity. The binding enthalpy of poly[d(A-T)] was found to be −5.5 Kcal/mol, which was much lower than any other polynucleotides, while the binding constant of the high affinity sites, was similar. In this report, the complete thermodynamic profiles of daunomycin binding to deoxypolynucleotides were reliably shown for the first time.

Keywords:

• Alternating sequences
• Anthracyclines
• Daunomycin
• Thermodynamic profiles

ACKNOWLEDGMENTS

This work was supported by a Korea Research Foundation Grant (KRF-1998-019-D00083) to D. S., by a Grant (KRF-2001-015-FP0128) to Y. O., by a Sundo Researcher Grant from the Korea Research Foundation (2000) & a Life Phenomena & Function Research Grant from the Korean Ministry of Science and Technology (2000) to M. H., and by a N.I.H. Grant CA35635 to J.B.C.