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Nucleosides, Nucleotides & Nucleic Acids Volume 22, 2003 - Issue 5-8
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Original Articles

Stabilization of DNA Double and Triple Helices by Conjugation of Minor Groove Binders to Oligonucleotides

A. S. Boutorine Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, INSERM U 565, CNRS UMR 8646, Paris Cedex, France; Laboratoire de Biophysique, Museum National d'Histoire Naturelle, 43, rue Cuvier, Paris Cedex 05, F-75231, FranceCorrespondence[email protected]
,
V. A. Ryabinin State Research Center for Virology and Biotechnology “Vector”, Novosibirsk Region, Russia
,
D. S. Novopashina Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Novosibirsk, Russia
,
A. G. Venyaminova Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Novosibirsk, Russia
,
C. Hélène Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, INSERM U 565, CNRS UMR 8646, Paris Cedex, France
&
A. S. Sinyakov State Research Center for Virology and Biotechnology “Vector”, Novosibirsk Region, Russia
Pages 1267-1272 | Published online: 31 Aug 2006
 
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Abstract

New conjugates containing two parallel or antiparallel carboxamide minor groove binders (MGB) attached to the same terminal phosphate of one oligonucleotide strand were synthesized. The conjugates interact with their target DNA stronger than the individual components. Effect of conjugated MGB on DNA duplex and triplex stability and their sequence specificity was demonstrated on the short oligonucleotide duplexes and on the triplex formed by model 16-mer oligonucleotide with HIV polypurine tract.

Acknowledgments

This work was supported by European Community (INTAS Open Project 01-0638) and French Ministry of Foreign Affairs (EGIDE 04542ND). We thank Mr. Ludovic Halby for excellent technical assistance.

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