Alkylation and Cleavage of a DNA Fragment with Trioligonucleotide Reagent

Abstract

A possibility of site‐directed chemical modification of a ssDNA fragment with “trioligonucleotide reagent” (TOR), consisting of a central oligonucleotide derivative carrying N‐(2‐chloroethyl)‐N‐(p‐formylphenyl)‐N‐propyl‐N‐3‐ydeneamino groups at both 5′‐ and 3′‐thiophosphate ends and two border derivatives with 4‐carbohydrazidephenyl groups at their 3′‐ and 5′‐phosphate ends, respectively, is shown. Products of site‐directed fragment cleavage, more abundant than the alkylation products, were found at 50°C. The overall level of DNA modification by TOR reached 30% at a small excess of the oligonucleotide derivatives.

Keywords:

• Oligonucleotide derivatives
• DNA alkylation
• Cleavage

Acknowledgments

This work was supported by the Russian Foundation for Basic Research, grant 02‐03‐32817.