The Influence of the Non‐Nucleotide Insert on the Hybridization Properties of Oligonucleotides

Abstract

The effect of different non‐nucleotide inserts incorporated into oligonucleotide chains on their hybridization properties was studied by the method of thermal denaturation. Various types of alkyldiols and oligoethylene glycols were used as inserts modifying oligonucleotide backbone. Such modification of oligonucleotides caused the destabilization of their complementary complexes. It was shown that the hybridization properties of the modified oligonucleotides depend on several features of inserts: the type, number, length of insertions, and positions of interrupted dinucleotide steps in oligonucleotide chain.

Keywords:

• Oligonucleotide
• Thermodynamics
• Non‐nucleotide insert
• Complex formation
• Antisense

Acknowledgments

This work was supported by grant of the program for young scientists of Siberian Division of RAS (#50), Grant of MCB Program of RAS (#10.6) and integration grant of SD RAS (#126).