MODULATION OF MACRONUTRIENTS UPTAKE AND TRANSLOCATION IN Mn-TREATED RICE IN EARLY STAGES OF VEGETATIVE GROWTH

Abstract

The effect of increasing manganese (Mn) concentrations on calcium (Ca), potassium (K), magnesium (Mg), sodium (Na), and phosphorus (P) absorption and translocation was studied in rice (Oryza sativa L. cv. Safari), before and after the end of mobilization of seed reserves. Rice plants were grown over a 15-, 21-, and 28-day period in nutrient solutions containing Mn concentrations varying between 0.125 and 32 mg L⁻¹. It was found that increasing Mn concentrations in the nutrient solution was coupled to an increasing net uptake, total shoot accumulation, and root and shoot contents of this metal during all the experimental periods. Concerning the translocation rates, in 15-day-old plants a decrease was found after the 2 mg L⁻¹ Mn treatment, but from the 21st day onward an increase was found until the highest treatment. The modulating action of Mn in macronutrient accumulation displayed different patterns among the experimental periods. In the root tissues of 15-day-old plants, Ca decreased significantly until the 2 mg L⁻¹ treatment and Na increased. In the shoots, the contents of P and Na decreased, but K and Mg showed significant increases. Until the 32 mg L⁻¹ Mn treatment, the ratio between root and shoot concentrations of K and Mg decreased in these plants. A similar pattern was also found for Ca until the 2 mg L⁻¹ Mn treatment. That ratio increased for Na. Plant total amount of Ca sharply decreased. Shoot total amount of Na and P also decreased, but the pattern of Ca increased until the 2 mg L⁻¹ Mn treatment. The concentrations of K increased in the root tissues 21 days after germination, but the levels of Ca, Mg, Na, and P decreased. In the shoots, the concentrations of Ca and Mg decreased significantly. Until the 32 mg L⁻¹ Mn treatment, the ratio between root and shoot concentration of Na and P increased, whereas those of Ca and Mg decreased. An increase was found for the plant and shoot total amount of Ca, K, and Mg until the last Mn treatment, but an opposite trend was found for Na and P. Additionally, until the 32 mg L⁻¹ Mn treatment, an increase was found for the proportions of Ca and Mg translocated to the shoot, but an opposite trend was detected for P. It was concluded that before and after the end of the mobilization of seed reserves, the net uptake rate of Ca is reversed, and, moreover, a similar trend is shown for the net translocation of Mg. A major implication of this process is the alteration of the related pattern for shoot accumulation. Eventually a different selectivity of the K⁺:Na⁺ ratio is also developed in the roots.

Acknowledgments