THE INTERACTION OF POLYSACCHARIDES WITH A SPECTROSCOPIC PROBE: THE ANION EFFECT ON THE BINDING SITE OF HEPARIN

Abstract

Heparin (Hep) has a variety of biological activities, most of them due to Hep's high anionic groups. The average binding number (n) is proposed to express the density of anion's group (binding site) on Hep. To gain insight into the binding site of Hep under the interference of anions, we have developed a method that makes use of isosbestic points in the reaction system in which two reagents compete for the same dye ion, and one complex is colorless. It is also necessary to determine the molar absorptivities of the various species concerned.

Keywords:

• Anion
• Binding site
• Heparin
• Azure A

Acknowledgments