¹³C Nuclear Magnetic Resonance Studies of the Conformations of Serine‐3′‐ and 5′‐Thymidine Monophosphate Conjugates

Abstract

The differences in the backbone conformation between O‐thymidine‐3′‐(1) and 5′‐yl O‐alkyl N‐phosphoryl serine methyl esters (2) have been investigated by solution ¹³C NMR spectroscopy. The stereo‐sensitive vicinal ³¹P–¹³C coupling constants were measured and used in the conformational analysis for the P–O5′–C5′, P–O3′–C3′, and P–N–C_α bonds. Three‐dimensional structural characteristics of dephosphorylation reactions of Compounds are also discussed.

Keywords:

• ¹³C NMR
• Conformation
• Dephosphorylation
• Serine‐thymidine
• Monophosphate conjugates

Acknowledgment

The authors would like to extend thanks for financial support from the Chinese National Natural Science Foundation (No. 20132020), the Ministry of Science and Technology, the Chinese Ministry of Education and Tsinghua University.