Abstract
A column-switching chromatographic system using the teicoplanin chiral stationary phase for stereoselective determination of the β-blocker (R,S)-propranolol in human plasma has been developed and validated. A conventional, off-line liquid–liquid extraction procedure was used for pretreatment of plasma samples and compared to an on-line sample preparation method employing a special type of packing material (LiChrospher RP-8 ADS). The HPLC-integrated sample pretreatment (on-line liquid–solid extraction) allows higher precision, accuracy, and sensitivity. Detailed validation parameters are discussed.
ACKNOWLEDGMENTS
The authors gratefully thank Professor Lindner for providing the human blood samples. We also thank Mrs Gyurkovicová for excellent technical assistance.