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Original Articles

Identification of Barley Varieties Used in Beer Production by Microsatellite DNA Markers

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Pages 47-51 | Published online: 01 Feb 2018
 

Abstract

Microsatellite DNA (or simple sequence repeat [SSR]) markers were used in this study for the identification of barley varieties. Seventeen SSRs were selected from a representative, highly informative genotyping set of barley SSRs. These SSRs detected polymorphism among 17 barley varieties imported by Tsingtao Brewery Co. Ltd. and used in its beer production. The number of alleles at different loci varied from two to five. Of 17 varieties, seven could be distinguished from each other and from the remaining varieties using specific alleles. As an example, the loci Bmag0125, Bmag0120, HVM62, and Bmag0353 in combination were able to distinguish all barley varieties tested. An allele-based retrieval system was developed for the 17 barley varieties based on these four SSRs.

RESUMEN

Los marcadores de microsatélite DNA (o secuencia simple de repetición [SSR]) fueron utilizados en este estudio para la identificación de las variedades de cebada. Diecisiete SSRs fueron seleccionados de un representante, altamente informativo sistema de genotipificación de cebada SSRs. Estos SSRs detectaron polimorfismo entre 17 variedades de cebada importadas de la cervecería de Tsingtao Co. Ltd. y usadas en su producción. El número de alelos en diversas posiciones varió a partir de dos a cinco. De 17 variedades, siete podían ser distinguidos entre unos de otros y de las variedades restantes usando los alelos específicos. Por ejemplo, las posiciones Bmag0125, Bmag0120, HVM62, y Bmag0353 en combinación podían distinguir todas las variedades de cebada probada. Un sistema de recuperación basado en alelos fue desarrollado para las 17 variedades de cebada basadas en estos cuatro SSRs.

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