Combined Action of Histone Reader Modules Regulates NuA4 Local Acetyltransferase Function but Not Its Recruitment on the Genome

Abstract

Recognition of histone marks by reader modules is thought to be at the heart of epigenetic mechanisms. These protein domains are considered to function by targeting regulators to chromosomal loci carrying specific histone modifications. This is important for proper gene regulation as well as propagation of epigenetic information. The NuA4 acetyltransferase complex contains two of these reader modules, an H3K4me3-specific plant homeodomain (PHD) within the Yng2 subunit and an H3K36me2/3-specific chromodomain in the Eaf3 subunit. While each domain showed a close functional interaction with the respective histone mark that it recognizes, at the biochemical level, genetic level (as assessed with epistatic miniarray profile screens), and phenotypic level, cells with the combined loss of both readers showed greatly enhanced phenotypes. Chromatin immunoprecipitation coupled with next-generation sequencing experiments demonstrated that the Yng2 PHD specifically directs H4 acetylation near the transcription start site of highly expressed genes, while Eaf3 is important downstream on the body of the genes. Strikingly, the recruitment of the NuA4 complex to these loci was not significantly affected. Furthermore, RNA polymerase II occupancy was decreased only under conditions where both PHD and chromodomains were lost, generally in the second half of the gene coding regions. Altogether, these results argue that methylated histone reader modules in NuA4 are not responsible for its recruitment to the promoter or coding regions but, rather, are required to orient its acetyltransferase catalytic site to the methylated histone 3-bearing nucleosomes in the surrounding chromatin, cooperating to allow proper transition from transcription initiation to elongation.

Supplemental material for this article may be found at http://dx.doi.org/10.1128/MCB.00112-16.

ACKNOWLEDGMENTS

We are grateful to Stéphane Allard, Andréanne Auger, Xue Cheng, and Nancy Lévesque for initial experiments/technical support in this project. We thank Amine Nourani for comments on the text.

M.J.A. was supported by a Frederick Banting and Charles Best Canada graduate scholarship. M.S.K. holds the Canada Research Chair in Social Epigenetics, and J.C. holds the Canada Research Chair in Chromatin Biology and Molecular Epigenetics.

Additional information

Funding

This work was funded by grants from the Canadian Institutes of Health Research (CIHR) to François Robert (MOP-82891), to Michael S. Kobor (MOP-119383), and to Jacques Côté (MOP-14308 and FDN-334002).