Abstract
T-cell-receptor (TCR)-mediated integrin activation is required for T-cell-antigen-presenting cell conjugation and adhesion to extracellular matrix components. While it has been demonstrated that the actin cytoskeleton and its regulators play an essential role in this process, no mechanism has been established which directly links TCR-induced actin polymerization to the activation of integrins. Here, we demonstrate that TCR stimulation results in WAVE2-ARP2/3-dependent F-actin nucleation and the formation of a complex containing WAVE2, ARP2/3, vinculin, and talin. The verprolin-connecting-acidic (VCA) domain of WAVE2 mediates the formation of the ARP2/3-vinculin-talin signaling complex and talin recruitment to the immunological synapse (IS). Interestingly, although vinculin is not required for F-actin or integrin accumulation at the IS, it is required for the recruitment of talin. In addition, RNA interference of either WAVE2 or vinculin inhibits activation-dependent induction of high-affinity integrin binding to VCAM-1. Overall, these findings demonstrate a mechanism in which signals from the TCR produce WAVE2-ARP2/3-mediated de novo actin polymerization, leading to integrin clustering and high-affinity binding through the recruitment of vinculin and talin.
We thank Tina Izard for the vinculin cDNA.
This work was supported by the Mayo Foundation and NIH grant R01-AI065474 to D.D.B., NIH grants R01-AI038474 and R01-AI031126 to Y.S., NIH grant R01-AI060921 to B.D.F., NIH-Cancer Biology Training Grant T32-CA009138 to J.S.M., and predoctoral Immunology Training Grant NIH-T32-AI07425 to J.C.N. D.D.B. is a Leukemia and Lymphoma Society Scholar.
We declare that we have no competing financial interests.