Abstract
Maintenance of cell type identity is crucial for health, yet little is known of the regulation that sustains the long-term stability of differentiated phenotypes. To investigate the roles that key transcriptional regulators play in adult differentiated cells, we examined the effects of depletion of the developmental master regulator PTF1A on the specialized phenotype of the adult pancreatic acinar cell in vivo. Transcriptome sequencing and chromatin immunoprecipitation sequencing results showed that PTF1A maintains the expression of genes for all cellular processes dedicated to the production of the secretory digestive enzymes, a highly attuned surveillance of unfolded proteins, and a heightened unfolded protein response (UPR). Control by PTF1A is direct on target genes and indirect through a ten-member transcription factor network. Depletion of PTF1A causes an imbalance that overwhelms the UPR, induces cellular injury, and provokes acinar metaplasia. Compromised cellular identity occurs by derepression of characteristic stomach genes, some of which are also associated with pancreatic ductal cells. The loss of acinar cell homeostasis, differentiation, and identity is directly relevant to the pathologies of pancreatitis and pancreatic adenocarcinoma.
Supplemental material for this article may be found at http://dx.doi.org/10.1128/MCB.00358-16.
ACKNOWLEDGMENTS
We thank Mark Borromeo, Rahul Kollipara, and Mei Jiang for assistance with bioinformatics, Jumin Xue for mouse husbandry and care, Jenna Jewell for help with phospho-RPS6 immunodetection, Steve Konieczny for MIST1 antibody, and Chaoying Liang, Quan-Zhen Li, and Ward Wakeland of the UT Southwestern Genomics and Microarray Core.