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Article

Acetylation of Rsc4p by Gcn5p Is Essential in the Absence of Histone H3 Acetylation

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Pages 6967-6972 | Received 08 Apr 2008, Accepted 12 Sep 2008, Published online: 27 Mar 2023
 

Abstract

Rsc4p, a subunit of the RSC chromatin-remodeling complex, is acetylated at lysine 25 by Gcn5p, a well-characterized histone acetyltransferase (HAT). Mutation of lysine 25 does not result in a significant growth defect, and therefore whether this modification is important for the function of the essential RSC complex was unknown. In a search to uncover the molecular basis for the lethality resulting from loss of multiple histone H3-specific HATs, we determined that loss of Rsc4p acetylation is lethal in strains lacking histone H3 acetylation. Phenotype comparison of mutants with arginine and glutamine substitutions of acetylatable lysines within the histone H3 tail suggests that it is a failure to neutralize the charge of the H3 tail that is lethal in strains lacking Rsc4p acetylation. We also demonstrate that Rsc4p acetylation does not require any of the known Gcn5p-dependent HAT complexes and thus represents a truly novel function for Gcn5p. These results demonstrate for the first time the vital and yet redundant functions of histone H3 and Rsc4p acetylation in maintaining cell viability.

ACKNOWLEDGMENTS

Support for this study was provided by a grant to L.J.H. from the Canadian Institutes of Health Research. L.J.H. is Canadian Institutes of Health Research New Investigator and a Scholar of the Michael Smith Foundation for Health Research.

We gratefully acknowledge Jacob Hodgson and Vicki MacDonald for critically reading the manuscript, Fred Winston for providing yeast strains and plasmids, and Song Tan and Patrick Grant for helpful discussions.

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