NSun2 Promotes Cell Growth via Elevating Cyclin-Dependent Kinase 1 Translation

Abstract

The tRNA methytransferase NSun2 promotes cell proliferation, but the molecular mechanism has not been elucidated. Here, we report that NSun2 regulates cyclin-dependent kinase 1 (CDK1) expression in a cell cycle-dependent manner. Knockdown of NSun2 decreased the CDK1 protein level, while overexpression of NSun2 elevated it without altering CDK1 mRNA levels. Further studies revealed that NSun2 methylated CDK1 mRNA in vitro and in cells and that methylation by NSun2 enhanced CDK1 translation. Importantly, NSun2-mediated regulation of CDK1 expression had an impact on the cell division cycle. These results provide new insight into the regulation of CDK1 during the cell division cycle.

ACKNOWLEDGMENTS

This work was supported by grants 81230008, 81420108016, and 91339114 from the National Science Foundation of China and by grant B07001 (111 project) from the Ministry of Education of People's Republic of China.

We declare that we have no conflicts of interest.