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Article

Differential Chromatin Looping Regulates CD4 Expression in Immature Thymocytes

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Pages 907-912 | Received 22 May 2007, Accepted 07 Nov 2007, Published online: 27 Mar 2023
 

Abstract

Runx1 binds the silencer and represses CD4 transcription in immature thymocytes. In this study, using looping chromatin immunoprecipitation and chromatin conformation capture assays, we demonstrated that interactions between Runx1 and positive elongation factor b (P-TEFb) appose the silencer and enhancer in CD4-negative thymoma cells and double-negative immature thymocytes. This chromatin loop decoys P-TEFb away from the promoter, thus preventing RNA polymerase II from elongating on the CD4 gene. In the absence of Runx1 on the silencer, P-TEFb interacts with the transcription complex, forming a different chromatin loop between the enhancer and the promoter, which leads to the expression of the CD4 gene in CD4-positive hybridoma cells and double-positive thymocytes. Moreover, the knockdown of CycT1 from P-TEFb abolishes both of these chromatin loops. Finally, the selective removal and restoration of Runx1 causes rapid interchanges between these chromatin loops, which reveals the plasticity of this regulatory circuit. Thus, differential looping and decoying of P-TEFb away from the promoter mediate active repression of the CD4 gene during thymocyte development.

ACKNOWLEDGMENTS

We thank Dan Littman and Takeshi Egawa for fruitful discussions and helping us to isolate primary thymocytes.

This work was supported by grants from the NIH and the Nora Eccles Treadwell Foundation.

We declare no conflict of interest with any aspects of this work.

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