Abstract
The nuclear and oncogenic BCL-3 protein activates or represses gene transcription when bound to NF-κB proteins p50 and p52, yet the molecules that specifically interact with BCL-3 and drive BCL-3-mediated effects on gene expression remain largely uncharacterized. Moreover, GSK3-mediated phosphorylation of BCL-3 triggers its degradation through the proteasome, but the proteins involved in this degradative pathway are poorly characterized. Biochemical purification of interacting partners of BCL-3 led to the identification of CtBP as a molecule required for the ability of BCL-3 to repress gene transcription. CtBP is also required for the oncogenic potential of BCL-3 and for its ability to inhibit UV-mediated cell apoptosis in keratinocytes. We also defined the E3 ligase TBLR1 as a protein involved in BCL-3 degradation through a GSK3-independent pathway. Thus, our data demonstrate that the LSD1/CtBP complex is required for the repressing abilities of an oncogenic IκB protein, and they establish a functional link between the E3 ligase TBLR1 and NF-κB.
This work was supported by grants from the FNRS, TELEVIE, the Belgian Federation against Cancer, the King Baudouin Foundation, the University of Liege (Concerted Research Action Program [04/09-323] and Fonds Spéciaux), the Inter-University Attraction Pole 6/12 (Federal Ministry of Science), the Centre Anti-Cancéreux, and the Leon Fredericq Foundation (ULg).
We are grateful to L. van Parijs, J. Mymryk, E. Olson, G. Gill, and E. Dejardin for the gifts of the pLL3.7 lentivirus, CtBP, CoREST, p52, p100, and p105 expression constructs. A.K. is a research assistant, P.C. and P.V. are senior research assistants, A.C. is a senior research associate, and K.S. is a temporary postdoctoral researcher at the Belgian National Funds for Scientific Research (FNRS).