ΔNp73 Modulates Nerve Growth Factor-Mediated Neuronal Differentiation through Repression of TrkA

Abstract

p73, a member of the p53 family, expresses two classes of proteins: the full-length TAp73 and the N-terminally truncated ΔNp73. While TAp73 possesses many p53-like features, ΔNp73 is dominant negative towards TAp73 and p53 and appears to have distinct functions in tumorigenesis and neuronal development. Given its biological importance, we investigated the role of ΔNp73 in nerve growth factor (NGF)-mediated neuronal differentiation in PC12 cells. We show that overexpression of ΔNp73α or ΔNp73β inhibits NGF-mediated neuronal differentiation in both p53-dependent and -independent manners. In line with this, we showed that the level of endogenous ΔNp73 is progressively diminished in differentiating PC12 cells upon NGF treatment and knockdown of ΔNp73 promotes NGF-mediated neuronal differentiation. Interestingly, we found that the ability of ΔNp73 to suppress NGF-mediated neuronal differentiation is correlated with its ability to regulate the expression of TrkA, the high-affinity NGF receptor. Specifically, we found that ΔNp73 directly binds to the TrkA promoter and transcriptionally represses TrkA expression, which in turn attenuates the NGF-mediated mitogen-activated protein kinase pathway. Conversely, the steady-state level of TrkA is increased upon knockdown of ΔNp73. Furthermore, we found that histone deacetylase 1 (HDAC1) and HDAC2 are recruited by ΔNp73 to the TrkA promoter and act as corepressors to suppress TrkA expression, which can be relieved by trichostatin A, an HDAC inhibitor. Taken together, we conclude that ΔNp73 negatively regulates NGF-mediated neuronal differentiation by transrepressing TrkA.

This work was supported in part by NIH grants CA081237 and CA102188.