SBA1 Encodes a Yeast Hsp90 Cochaperone That Is Homologous to Vertebrate p23 Proteins

ABSTRACT

The Saccharomyces cerevisiae SBA1 gene was cloned by PCR amplification from yeast genomic DNA following its identification as encoding an ortholog of human p23, an Hsp90 cochaperone. TheSBA1 gene product is constitutively expressed and nonessential, although a disruption mutant grew more slowly than the wild type at both 18 and 37°C. A double deletion of SBA1and STI1, encoding an Hsp90 cochaperone, displayed synthetic growth defects. Affinity isolation of histidine-tagged Sba1p (Sba1^His6) after expression in yeast led to coisolation of Hsp90 and the cyclophilin homolog Cpr6. Using an in vitro assembly assay, purified Sba1^His6 bound to Hsp90 only in the presence of adenosine 5′-O-(3-thiotriphosphate) or adenyl-imidodiphosphate. Furthermore, interaction between purified Sba1^His6 and Hsp90 in yeast extracts was inhibited by the benzoquinoid ansamycins geldanamycin and macbecin. The in vitro assay was also used to identify residues in Hsp90 that are important for complex formation with Sba1^His6, and residues in both the N-terminal nucleotide binding domain and C-terminal half were characterized. In vivo analysis of known Hsp90 substrate proteins revealed that Sba1 loss of function had only a mild effect on the activity of the tyrosine kinase v-Src and steroid hormone receptors.

ACKNOWLEDGMENTS

We thank Jie Jin for help with protein purification, Alex Shorstein for help with SBA1 gene cloning, and Robert J. Donnelly (Molecular Resource Facility, New Jersey Medical School, UMDNJ) for DNA sequencing. We also thank F. Boschelli and E. Craig, Y. Kimura, S. Lindquist, D. Picard, D. Toft, and K. Yamamoto for the gifts of reagents, Sean Bohen for discussions and communication of results prior to publication, and Jeanne Hirsch for comments on the manuscript.

This work was supported by grants from the NIH (R01-DK49065) and the Sinsheimer Foundation to A.J.C.