Role of Phosphatidylinositol 4,5-Bisphosphate in Ras/Rac-Induced Disruption of the Cortactin-Actomyosin II Complex and Malignant Transformation

ABSTRACT

Oncogenic Ras mutants such as v-Ha-Ras cause a rapid rearrangement of actin cytoskeleton during malignant transformation of fibroblasts or epithelial cells. Both PI-3 kinase and Rac are required for Ras-induced malignant transformation and membrane ruffling. However, the signal transduction pathway(s) downstream of Rac that leads to membrane ruffling and other cytoskeletal change(s) as well as the exact biochemical nature of the cytoskeletal change remain unknown. Cortactin/EMS1 is the first identified molecule that is dissociated in a Rac–phosphatidylinositol 4,5-biphosphate (PIP₂)-dependent manner from the actin-myosin II complex during Ras-induced malignant transformation; either the PIP₂ binder HS1 or the Rac blocker SCH51344 restores the ability of EMS1 to bind the complex and suppresses the oncogenicity of Ras. Furthermore, while PIP₂ inhibits the actin-EMS1 interaction, HS1 reverses the PIP₂ effect. Thus, we propose that PIP₂, an end-product of the oncogenic Ras/PI-3 kinase/Rac pathway, serves as a second messenger in the Ras/Rac-induced disruption of the actin cytoskeleton and discuss the anticancer drug potential of PIP₂-binding molecules.

ACKNOWLEDGMENTS

We are grateful to Rob Michalides for his gift of human EMS1 cDNA, Henk van Damme and Vera van Buuren for their studies on intracellular localization of EMS1, Anjali Tikoo for her unpublished information on the anti-Ras action of F-actin cappers and cofilin mutants, and Tony Burgess for his consistent support and encouragement throughout this study.

The work has been supported in part by a grant from the Anti-Cancer Council of Victoria.