Abstract
In mammalian systems, the heterodimeric basic helix-loop-helix (bHLH)-PAS transcription hypoxia-inducible factor (HIF) has emerged as the key regulator of responses to hypoxia. Here we define a homologous system in Drosophila melanogaster, and we characterize its activity in vivo during development. By using transcriptional reporters in developing transgenic flies, we show that hypoxia-inducible activity rises to a peak in late embryogenesis and is most pronounced in tracheal cells. We show that the bHLH-PAS proteins Similar (Sima) and Tango (Tgo) function as HIF-α and HIF-β homologues, respectively, and demonstrate a conserved mode of regulation for Sima by oxygen. Sima protein, but not its mRNA, was upregulated in hypoxia. Time course experiments following pulsed ectopic expression demonstrated that Sima is stabilized in hypoxia and that degradation relies on a central domain encompassing amino acids 692 to 863. Continuous ectopic expression overrode Sima degradation, which remained cytoplasmic in normoxia, and translocated to the nucleus only in hypoxia, revealing a second oxygen-regulated activation step. Abrogation of the Drosophila Egl-9 prolyl hydroxylase homologue, CG1114, caused both stabilization and nuclear localization of Sima, indicating a central involvement in both processes. Tight conservation of the HIF/prolyl hydroxylase system in Drosophila provides a new focus for understanding oxygen homeostasis in intact multicellular organisms.
We thank Andrea Brand, Steve Crews, Shigeo Hayashi, Luis Quesada-Allué, Ricardo Ramos, Benny Shilo, Carl Thummel, the Bloomington Stocks Center, and the Developmental Studies Hybridoma Bank for plasmids, antibodies, and fly stocks. We thank Benny Shilo and Osvaldo Podhajcer for critical reading of the manuscript.
This work was supported by the Wellcome Trust grant HHBR6 and Fundación Antorchas grant A-13740/1-119 to P.W. and P.J.R. S.L.-L. and D.M.R. are fellows of the Argentinean National Council of Scientific Research (CONICET), S.N.B. received a postdoctoral fellowship from The Wellcome Trust, L.C. is a fellow of the FONCyT, M.I. and M.M. have FOMEC fellowships, and P.W. is a career investigator of CONICET.