Specific Inhibition of Elm1 Kinase Activity Reveals Functions Required for Early G₁ Events

Abstract

In budding yeast, the Elm1 kinase is required for coordination of cell growth and cell division at G₂/M. Elm1 is also required for efficient cytokinesis and for regulation of Swe1, the budding yeast homolog of the Wee1 kinase. To further characterize Elm1 function, we engineered an ELM1 allele that can be rapidly and selectively inhibited in vivo. We found that inhibition of Elm1 kinase activity during G₂ results in a phenotype similar to the phenotype caused by deletion of the ELM1 gene, as expected. However, inhibition of Elm1 kinase activity earlier in the cell cycle results in a prolonged G₁ delay. The G₁ requirement for Elm1 kinase activity occurs before bud emergence, polarization of the septins, and synthesis of G₁ cyclins. Inhibition of Elm1 kinase activity during early G₁ also causes defects in the organization of septins, and inhibition of Elm1 kinase activity in a strain lacking the redundant G₁ cyclins CLN1 and CLN2 is lethal. These results demonstrate that the Elm1 kinase plays an important role in G₁ events required for bud emergence and septin organization.

View correction statement:

Specific Inhibition of Elm1 Kinase Activity Reveals Functions Required for Early G1 Events

ACKNOWLEDGMENTS

We thank Eric Weiss, David Drubin, Jeff Ubersax, and David Morgan for helpful advice and/or sharing unpublished results. We also thank Stephanie Anastasia for help with experiments.

This work was supported by the Pew Biomedical Scholars program and by a grant from the National Institutes of Health.