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DNA Dynamics and Chromosome Structure

Systematic Mutagenesis of the Saccharomyces cerevisiae MLH1 Gene Reveals Distinct Roles for Mlh1p in Meiotic Crossing Over and in Vegetative and Meiotic Mismatch Repair

, , , , &
Pages 873-886 | Received 30 Aug 2002, Accepted 06 Nov 2002, Published online: 27 Mar 2023
 

Abstract

In eukaryotic cells, DNA mismatch repair is initiated by a conserved family of MutS (Msh) and MutL (Mlh) homolog proteins. Mlh1 is unique among Mlh proteins because it is required in mismatch repair and for wild-type levels of crossing over during meiosis. In this study, 60 new alleles of MLH1 were examined for defects in vegetative and meiotic mismatch repair as well as in meiotic crossing over. Four alleles predicted to disrupt the Mlh1p ATPase activity conferred defects in all functions assayed. Three mutations, mlh1-2, -29, and -31, caused defects in mismatch repair during vegetative growth but allowed nearly wild-type levels of meiotic crossing over and spore viability. Surprisingly, these mutants did not accumulate high levels of postmeiotic segregation at the ARG4 recombination hotspot. In biochemical assays, Pms1p failed to copurify with mlh1-2, and two-hybrid studies indicated that this allele did not interact with Pms1p and Mlh3p but maintained wild-type interactions with Exo1p and Sgs1p. mlh1-29 and mlh1-31 did not alter the ability of Mlh1p-Pms1p to form a ternary complex with a mismatch substrate and Msh2p-Msh6p, suggesting that the region mutated in these alleles could be responsible for signaling events that take place after ternary complex formation. These results indicate that mismatches formed during genetic recombination are processed differently than during replication and that, compared to mismatch repair functions, the meiotic crossing-over role of MLH1 appears to be more resistant to mutagenesis, perhaps indicating a structural role for Mlh1p during crossing over.

ACKNOWLEDGMENTS

We thank members of the Alani laboratory, Neil Hunter, Mike Liskay, and Wei Yang for helpful discussions and comments on the manuscript, Jennifer Wanat for analysis of SK1/S288C hybrid strains, and Rhona Borts for sharing unpublished data. We are grateful to M. Hall and T. Kunkel for providing the MLH1 and PMS1 overexpression vectors, H. Tsubouchi and H. Ogawa for providing the parental SK1 strains, and R. Lahue for anti-Pms1p antibody.

E.A. and A.W.K. were supported by National Institutes of Health grant GM53085. J.L.A. was supported by a CAPES fellowship awarded by the Brazilian government. J.H. was supported by a Department of Education training grant. M.W. and S.S. were funded by undergraduate research fellowships from the Howard Hughes Medical Institute awarded to Cornell University. M.W. was also supported by a Cornell Presidential Undergraduate Scholarship.

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