Severe Adenine Starvation Activates Ty1 Transcription and Retrotransposition in Saccharomyces cerevisiae

Abstract

Ty1 retrotransposons of the yeast Saccharomyces cerevisiae are activated by different kinds of stress. Here we show that Ty1 transcription is stimulated under severe adenine starvation conditions. The Bas1 transcriptional activator, responsible for the induction of genes of the de novo AMP biosynthesis pathway (ADE) in the absence of adenine, is not involved in this response. Activation occurs mainly on Ty1 elements, whose expression is normally repressed by chromatin and is suppressed in a hta1-htb1Δ mutant that alters chromatin structure. Activation is also abolished in a snf2Δ mutant. Several regions of the Ty1 promoter are necessary to achieve full activation, suggesting that full integrity of the promoter sequences might be important for activation. Together, these observations are consistent with a model in which the activation mechanism involves chromatin remodeling at Ty1 promoters. The consequence of Ty1 transcriptional activation in response to adenine starvation is an increase in Ty1 cDNA levels and a relief of Ty1 dormancy. The retrotransposition of four native Ty1 elements increases in proportion to their increase in transcription. Implications for the regulation of Ty1 mobility by changes in Ty1 mRNA levels are discussed.

ACKNOWLEDGMENTS

We are grateful to B. Daignan-Fornier, B. Pinson, L. Benard, and C. Condon for stimulating discussions and helpful comments on the manuscript. We are grateful to J. Curcio, G. Fink, and B. Daignan-Fornier for providing strains and plasmids.

This work was supported by grants from the CNRS (UPR 9073), the Université Paris VII, and the Association pour la Recherche contre le Cancer (grant number 4663 to P.L.). A.-L.T. was a recipient of a fellowship from the Ministère pour la Recherche et la Technologie and of a fellowship from the Association pour la Recherche contre le Cancer.