Transfected Human β-Polymerase Promoter Contains a ras-Responsive Element

Abstract

β-Polymerase is a vertebrate cellular DNA polymerase involved in gap-filling synthesis during some types of genomic DNA repair. We report that a cloned human β-polymerase promoter in a transient expression assay is activated by p21^v-ras expression in NIH 3T3 cells. A decanucleotide palindromic element, GTGACGTCAC, at positions –49 to –40 in the promoter is required for this ras-mediated stimulation.