Cloning of a Growth Arrest-Specific and Transforming Growth Factor β-Regulated Gene, TI 1, from an Epithelial Cell Line

Abstract

By cDNA cloning and differential screening, five genes that are regulated by transforming growth factor β (TGFp) in mink lung epithelial cells were identified. A novel membrane protein gene, TI 1, was identified which was downregulated by TGFβand serum in quiescent cells. In actively growing cells, the TI 1 gene is rapidly and transiently induced by TGFβ, and it is overexpressed in the presence of protein synthesis inhibitors. It appears to be related to a family of transmembrane glycoproteins that are expressed on lymphocytes and tumor cells. The four other genes were all induced by TGFβand correspond to the genes of collagen α type I, fibronectin, plasminogen activator inhibitor 1, and the monocyte chemotactic cell-activating factor (JE gene) previously shown to be TGFβregulated. ^{* †}