Primary Structure of the Nuclear PUT2 Gene Involved in the Mitochondrial Pathway for Proline Utilization in Saccharomyces cerevisiae

Abstract

The PUT2 gene, believed to encode δ¹-pyrroline-5-carboxylate dehydrogenase, has been completely sequenced. The DNA contains an open reading frame of 1,725 base pairs encoding a protein of 575 amino acids. Transcript mapping with both S1 nuclease and primer extension methods revealed numerous initiation sites of RNA synthesis 50 to 80 base pairs downstream from several TATA boxes. The deduced amino acid sequence of δ¹-pyrroline-5-carboxylate dehydrogenase contains a highly basic amino terminus that may serve as the signal sequence that targets this protein to the mitochondrion.