Identification of a Yeast Protein with Properties Similar to Those of the Immunoglobulin Heavy-Chain Enhancer-Binding Protein NF-μE3

Abstract

We demonstrate that Saccharomyces cerevisiae cells possess a 33-41-kilodalton protein with DNA-binding properties remarkably similar to those of the immunoglobulin enhancer-binding protein NF-μE3. We further show that the μE3-binding site functions as an upstream activating sequence in yeast cells, stimulating transcription from a truncated CYC1 promoter. These data suggest that the yeast protein, designated YEB-3, and NF-μE3 are functionally related and perhaps evolutionarily conserved.