In Vitro Degradation and Drug-Release Properties of Water-Soluble Chitosan Cross-Linked Oxidized Sodium Alginate Core–Shell Microgels

Abstract

Hydrogels based on sodium alginate (SA) have already been widely used in biomedical applications using Ca²⁺ as a cross-linker; however, these hydrogels tend to disintegrate in electrolyte solutions. To solve this problem, we present a kind of oxidized sodium alginate (OSA) microgel using water-soluble chitosan (WSC) as a cross-linker. This microgel was successfully prepared via an emulsion cross-linking technique at room temperature. The microgel was cross-linked by the formation of both Schiff base bonds and inter-polyelectrolyte complexes, which can efficiently eliminate the disintegration of the microgel in electrolyte solutions. Morphological properties of the resulting microgels were determined by transmission electron microscopy (TEM), hydrodynamic diameters of the microgels were characterized by dynamic light scattering (DLS). The objective of this work was to achieve the colon-specific delivery of an anti-ulcerative colitis drug. 5-Aminosalicylic acid (5-ASA) was chosen as a model drug and the in vitro drug-release profile was established in buffer solutions with 0.1 M HCl/NaCl (pH 1.2) and 0.1 M phosphate-buffered saline (PBS, pH 7.4) at 37°C. The microgel was incubated in 0.1 M PBS (pH 7.4) at 37°C to determine its degradation behavior. Cell cytotoxicity (tested by MTT assay) showed that this microgel had no significant cytotoxicity. These results indicated that this microgel prepared by introducing WSC into OSA may have potential applications in oral controlled drug-delivery systems. Therefore, the OSA/WSC microgel may be a useful carrier for the colon-specific delivery of anti-inflammatory drugs including 5-ASA and the enhanced therapeutic effect of ulcerative colitis.

Keywords:

• Chitosan
• sodium alginate
• microgel
• core-shell
• 5-aminosalicyh’c acid

Acknowledgements

We gratefully acknowledge the financial support of the Special Doctorial Program Fund of the Ministry of Education of China (Grant No. 20090211110004) and Gansu Province Project of Science and Technologies (Grant No. 0804WCGA130).