Modelling the kinetics of antigen-antibody reactions at particle enhanced optical immunoassays

Abstract

Functionalized latexes coated by antibodies are used in diagnostic tests for the detection of antigens in biological fluids. A simple kinetic model is presented which is related to the optical monitoring of the formation of specific complexes between antigen and antibody amplified by latex beads. The antibodies are chemically coupled onto chloromethylstyrene (CMST) particles. The kinetic model is able to describe the immunoprecipitin curves of immunolatex beads. The number of fitting parameters is relatively reduced (only three), and the meaning of these parameters can be interpreted in terms of the chemical equilibrium constant, the percentage of active IgG on the latex beads, and optical response. The model explains very well the optical response of immunolatex prepared by covalent coupling of antibodies on polymer carriers.

Keywords:

• Kev words: Reaction kinetics
• functionalized latex
• covalent IgG coupling
• enhanced-particle optical immunoassays
• reactivity of IgG
• IgG adsorbed at interfaces
• latex reagents