Effect of carbon-coated polyethylene terephthalate on prostacyclin release by endothelial cells stimulated with arachidonic acid in vitro

Abstract

The production of 6-kcto prosta-landin F_lα (6-keto-PGF_1α), stable metabolyte of prostacyclin, by cultured human endothelial cells in contact with carbon- and collagen-coated polyethylene terephthalate (PC), was assessed by enzyme immunoassay. As control material, tissue culture-treated polystyrene was used. The cultures were put in contact with the materials for 48 h and then were stimulated with 0.1 mM arachidonic acid for 3 h. The stimulation induced a highly signilicant increase of 6-keto-PGF _1α in the cultures in contact with the control material. PC induced only insignificant variations in stimulated cultures compared to unstimulated ones. In conclusion, PC determined a decrease in the endothelial cell response to stimulation with arachidonic acid.

Keywords:

• Prostacyclin
• endothelial cell
• carbon
• polyethylene terephthalate