Effect of dialysis on oxidative stress in uraemia

Abstract

It has been postulated that dialysis of patients with chronic renal failure (CRF) is associated with increased lipid peroxidation which may contribute to vascular and other complications of the syndrome. In the present study, a specific and precise technique [ferrous oxidation in xylenol orange (FOX) assay] was used to measure plasma lipid hydroperoxides (ROOHs) in three groups of uraemic patients. Patients were either studied before starting dialysis (n= 12) or on continuous ambulatory peritoneal dialysis (CAPD, n= 12) or haemodialysis (HD, n= 36) and compared to healthy controls (n=20). Plasma ROOHs were markedly elevated in HD patients compared with the controls (7.01±2.9 µM versus 4.25±2.05 µM; P < 0.005, Mann-Whitney test). Plasma ROOH concentrations in the CAPD patients were increased but not significantly higher than controls (5.36±3.56 µM versus 4.25±2.05 µM). By contrast, no differences in ROOH levels were found between controls and predialysis patients. There was no difference in plasma thiobarbituric acid reactive substances (TBARS)between control and the three CRF groups. Absolute and cholesterol standardised plasma α-tocopherol levels were lower in the patients (whether they were on dialysis or not) than in the controls (18.62±6.88 µM versus 22.73±5.33 µM; P < 0.01 and 1.99±1.88 µM/mM versus 5.25±1.0 µM/mM; P < 0.0005, respectively). This study provides direct evidence that enhanced oxidative stress in CRF patients is related to the dialysis treatment rather than the disease itself. Further studies will be necessary to establish the relationships between plasma measures of oxidative stress and cardiovascular complications in CRF patients under dialysis and whether treatment with antioxidants may reduce oxidative stress or reverse adverse effects associated with dialysis.