Abstract
Background/aims: Global cerebral ischemia/reperfusion (GCIR) may incur neurocognitive impairment. Tea polyphenols (TP) have strong anti-oxidant capacity. This study planned to investigate the protective effect of TP against the neurocognitive impairment caused by GCIR and its mechanism.
Methods: One-stage anterior approach for cerebral four-vessel occlusion (4VO) was used to construct the GCIR model. Sprague Dawley rats were randomly classified into Sham group, GCIR group, and TP group (n = 50 per group). Besides receiving the same 4VO, the rats in TP group were treated with TP (6.4%) injection from the tail vein 30 minutes before cerebral ischemia. Morris water-maze test was used to evaluate the changes in space recognition and memory and open field activity test to assess the activity and motor function of rats. The cell apoptotic study in hippocampal CA1 region at specified time points (12, 24, 48, and 72 hours after surgery) was carried out by the flow cytometry, histology (hematoxylin and eosin staining), and immunohistochemical (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling staining) examinations. One-way analysis of variance and least significant difference t-test were used and statistical significance considered at P < 0.05.
Results: Compared with the GCIR group, the TP group was significantly attenuated in the impairment of space recognition and memory caused by GCIR and so was the neuronal apoptosis in the hippocampal CA1 region (P < 0.05).
Conclusion: TP may attenuate the impairment of space recognition and memory caused by GCIR via anti-apoptosis.
Acknowledgment
This study was supported by grants from the National Natural Science Foundation of China (No: 81471131, 81470968, 81100530).
Disclaimer statements
Contributors
R.-L.X. and X.L. conceived and designed the experiments; G.W., X.W., J.L., R.F., and Z.Z. performed the experiments; W.L. and J. analyzed the data; H.Z., J.Z., and X.D. contributed reagents/materials/analysis tools, and R.-L.X. wrote the manuscript.
Funding
None.
Conflicts of interest
None.
Ethics approval
Ethics Committee of the Second Affiliated Hospital of Xi'an Jiaotong University approved animal use for this study.